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Preparación y análisis morfológico de cultivos de células de cresta neural craneal de pollo
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Developmental Biology
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JoVE Journal Developmental Biology
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
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Preparación y análisis morfológico de cultivos de células de cresta neural craneal de pollo

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2,216 Views

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09:26 min

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June 27, 2022

DOI:

10.3791/63799-v

DOI:
10.3791/63799-v

•

09:26 min
June 27, 2022

•

9 Views
, , ,
1Department of Biology and Neuroscience Program,Hamline University, 2Department of Genetics, Cell Biology and Development,University of Minnesota, 3Developmental Biology Center,University of Minnesota

Summary

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Este protocolo versátil describe el aislamiento de células de la cresta neural premigratoria (NCC) a través de la escisión de pliegues neurales craneales de embriones de pollo. Tras el recubrimiento y la incubación, las NCC migratorias emergen de los explantes del pliegue neural, lo que permite la evaluación de la morfología celular y la migración en un entorno 2D simplificado.

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Cite this Article

Jacques-Fricke, B. T., Roffers-Agarwal, J., Gustafson, C. M., Gammill, L. S. Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures. J. Vis. Exp. (184), e63799, doi:10.3791/63799 (2022).

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