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Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
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发育生物学
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JoVE 杂志 发育生物学
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

2,226 Views

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09:26 min

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June 27, 2022

DOI:

10.3791/63799-v

DOI:
10.3791/63799-v

•

09:26 min
June 27, 2022

•

9 Views
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1Department of Biology and Neuroscience Program,Hamline University, 2Department of Genetics, Cell Biology and Development,University of Minnesota, 3Developmental Biology Center,University of Minnesota

Summary

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This versatile protocol describes the isolation of premigratory neural crest cells (NCCs) through the excision of cranial neural folds from chick embryos. Upon plating and incubation, migratory NCCs emerge from neural fold explants, allowing for assessment of cell morphology and migration in a simplified 2D environment.

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Cite this Article

Jacques-Fricke, B. T., Roffers-Agarwal, J., Gustafson, C. M., Gammill, L. S. Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures. J. Vis. Exp. (184), e63799, doi:10.3791/63799 (2022).

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