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发育生物学
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Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
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发育生物学
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JoVE 杂志 发育生物学
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

DOI:
10.3791/63799-v

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09:26 min

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June 27, 2022

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, , ,
1Department of Biology and Neuroscience Program,Hamline University, 2Department of Genetics, Cell Biology and Development,University of Minnesota, 3Developmental Biology Center,University of Minnesota

Chapters

  • 00:04Introduction
  • 01:06Embryo Incubation and Preparation of Culture Dishes
  • 01:49Isolation of Chick Embryos
  • 03:01Dissecting Neural Folds
  • 04:22Plating Neural Folds
  • 05:20Fixing and Staining of Cultured Migratory Neural Crest Cells for Morphological Analysis
  • 07:08Results: Assessment of Cultured Migratory Neural Crest Cells
  • 08:39Conclusion

Summary

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This versatile protocol describes the isolation of premigratory neural crest cells (NCCs) through the excision of cranial neural folds from chick embryos. Upon plating and incubation, migratory NCCs emerge from neural fold explants, allowing for assessment of cell morphology and migration in a simplified 2D environment.

Tags

Keywords: Neural Crest CellsCranial Neural Fold CultureChick EmbryoNeural Crest MigrationMorphometric AnalysisFibronectinRinger’s SolutionCell DissociationPrimary Migratory Cells

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