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Voorbereiding en morfologische analyse van Chick Cranial Neural Crest Cell Cultures
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Developmental Biology
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JoVE Journal Developmental Biology
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
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Voorbereiding en morfologische analyse van Chick Cranial Neural Crest Cell Cultures

Nederlands

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2,226 Views

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09:26 min

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June 27, 2022

DOI:

10.3791/63799-v

DOI:
10.3791/63799-v

•

09:26 min
June 27, 2022

•

9 Views
, , ,
1Department of Biology and Neuroscience Program,Hamline University, 2Department of Genetics, Cell Biology and Development,University of Minnesota, 3Developmental Biology Center,University of Minnesota

Summary

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Dit veelzijdige protocol beschrijft de isolatie van premigratory neural crest cells (NCC's) door de excisie van craniale neurale plooien van kuikenembryo's. Bij plating en incubatie komen migrerende NCC's tevoorschijn uit neurale vouwexplantaten, waardoor celmorfologie en migratie in een vereenvoudigde 2D-omgeving kunnen worden beoordeeld.

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Cite this Article

Jacques-Fricke, B. T., Roffers-Agarwal, J., Gustafson, C. M., Gammill, L. S. Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures. J. Vis. Exp. (184), e63799, doi:10.3791/63799 (2022).

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