Journal
/
Biología del desarrollo
/
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

2,226 Views

•

09:26 min

•

June 27, 2022

DOI:

10.3791/63799-v

DOI:
10.3791/63799-v

•

09:26 min
June 27, 2022

•

9 Views
, , ,
1Department of Biology and Neuroscience Program,Hamline University, 2Department of Genetics, Cell Biology and Development,University of Minnesota, 3Developmental Biology Center,University of Minnesota

Summary

Automatically generated

This versatile protocol describes the isolation of premigratory neural crest cells (NCCs) through the excision of cranial neural folds from chick embryos. Upon plating and incubation, migratory NCCs emerge from neural fold explants, allowing for assessment of cell morphology and migration in a simplified 2D environment.

Explore More Videos

Neural Crest CellsCranial Neural Fold CultureChick EmbryoNeural Crest MigrationMorphometric AnalysisFibronectinRinger’s SolutionCell DissociationPrimary Migratory Cells

Read Article

Cite this Article

Jacques-Fricke, B. T., Roffers-Agarwal, J., Gustafson, C. M., Gammill, L. S. Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures. J. Vis. Exp. (184), e63799, doi:10.3791/63799 (2022).

Download .ris file

Copy

Share Video

.

Copy