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Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction
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Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction

Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction

DOI:
10.3791/63742-v

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05:33 min

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July 26, 2022

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1Electron Microscopy Laboratory, Faculty of Mechanical Engineering,Wroclaw University of Science and Technology, 2Advanced Materials Engineering and Modelling Group, Faculty of Chemistry,Wroclaw University of Science and Technology

Chapters

  • 00:04Introduction
  • 00:41Encapsulation of Bacteria with the Photosensitizer
  • 02:07In Situ TEM Observations of Bacterial Cells in Liquid–Electron Dose Effect
  • 02:50In Situ TEM Observations of Light‐Induced Processes Between Bacterial Cells and Photosensitizer
  • 03:51Results: TEM Images of S. aureus Encapsulated with Methylene Blue Between Two Carbon Films and the Unfavorable Effects That May Occur During Imaging
  • 04:47Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes transmission electron microscope (TEM) modifications with a light illumination system, the fabrication of liquid cells, and in situ TEM observations of light-induced interactions between bacterial cells and a photosensitizer. The sample preparation methods, electron beam damage, and imaging are also discussed.

Tags

Keywords: Light-inducedIn SituTransmission Electron MicroscopyLiquid-soft Matter InteractionBacteriaPhotosensitizerSample PreparationLiquid Cell TEMElectron DoseLaser Light Intensity

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