00:41Encapsulation of Bacteria with the Photosensitizer
02:07In Situ TEM Observations of Bacterial Cells in Liquid–Electron Dose Effect
02:50In Situ TEM Observations of Light‐Induced Processes Between Bacterial Cells and Photosensitizer
03:51Results: TEM Images of S. aureus Encapsulated with Methylene Blue Between Two Carbon Films and the Unfavorable Effects That May Occur During Imaging
The present protocol describes transmission electron microscope (TEM) modifications with a light illumination system, the fabrication of liquid cells, and in situ TEM observations of light-induced interactions between bacterial cells and a photosensitizer. The sample preparation methods, electron beam damage, and imaging are also discussed.