The present protocol describes transmission electron microscope (TEM) modifications with a light illumination system, the fabrication of liquid cells, and in situ TEM observations of light-induced interactions between bacterial cells and a photosensitizer. The sample preparation methods, electron beam damage, and imaging are also discussed.
Żak, A., Kaczmarczyk, O. Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction. J. Vis. Exp. (185), e63742, doi:10.3791/63742 (2022).