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Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction
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Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction

Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction

1,944 Views

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05:33 min

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July 26, 2022

DOI:

10.3791/63742-v

DOI:
10.3791/63742-v

•

05:33 min
July 26, 2022

•

7 Views
,
1Electron Microscopy Laboratory, Faculty of Mechanical Engineering,Wroclaw University of Science and Technology, 2Advanced Materials Engineering and Modelling Group, Faculty of Chemistry,Wroclaw University of Science and Technology

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The present protocol describes transmission electron microscope (TEM) modifications with a light illumination system, the fabrication of liquid cells, and in situ TEM observations of light-induced interactions between bacterial cells and a photosensitizer. The sample preparation methods, electron beam damage, and imaging are also discussed.

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Light-inducedIn SituTransmission Electron MicroscopyLiquid-soft Matter InteractionBacteriaPhotosensitizerSample PreparationLiquid Cell TEMElectron DoseLaser Light Intensity

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Żak, A., Kaczmarczyk, O. Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction. J. Vis. Exp. (185), e63742, doi:10.3791/63742 (2022).

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