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Een geïntegreerde workflow van identificatie en kwantificering op FDR Control-Based Untargeted Metabolome
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Biochemistry
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JoVE Journal Biochemistry
An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
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Een geïntegreerde workflow van identificatie en kwantificering op FDR Control-Based Untargeted Metabolome

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3,473 Views

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05:35 min

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September 20, 2022

DOI:

10.3791/63625-v

DOI:
10.3791/63625-v

•

05:35 min
September 20, 2022

•

3 Views
, , ,
1Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and MOE Key Laboratory of Tumor Molecular Biology, Institute of Life and Health Engineering, College of Life Science and Technology,Jinan University, 2Chi-Biotech Co. Ltd.

Summary

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We bouwden een ongerichte metabolomische workflow die XY-Meta en metaX samen integreerde. In dit protocol hebben we laten zien hoe we XY-Meta kunnen gebruiken om een lokspectrale bibliotheek te genereren uit open access spectrareferentie, en vervolgens FDR-controle uitgevoerd en de metaX gebruikt om de metabolieten te kwantificeren na het identificeren van de metabolomics-spectra.

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Cite this Article

Li, D., Liang, J., Zhang, Y., Zhang, G. An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome. J. Vis. Exp. (187), e63625, doi:10.3791/63625 (2022).

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