ジャーナル
/
生化学
/
An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

3,471 Views

•

05:35 min

•

September 20, 2022

DOI:

10.3791/63625-v

DOI:
10.3791/63625-v

•

05:35 min
September 20, 2022

•

3 Views
, , ,
1Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and MOE Key Laboratory of Tumor Molecular Biology, Institute of Life and Health Engineering, College of Life Science and Technology,Jinan University, 2Chi-Biotech Co. Ltd.

概要

Automatically generated

We constructed an untargeted metabolomic workflow that integrated XY-Meta and metaX together. In this protocol, we displayed how to use XY-Meta to generate a decoy spectral library from open access spectra reference, and then performed FDR control and used the metaX to quantitate the metabolites after identifying the metabolomics spectra.

Explore More Videos

FDR ControlUntargeted MetabolomeXY-MetaTarget-decoy StrategyMetabolite IdentificationFalse Positive ReductionBiomarker DiscoveryGNPS DatabaseProteoWizardRaw Data ConversionNIST LibraryXY-Meta ProgramMetaX SoftwareQuantitative AnalysisPCA PlotPeak Annotation

Read Article

Cite this Article

Li, D., Liang, J., Zhang, Y., Zhang, G. An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome. J. Vis. Exp. (187), e63625, doi:10.3791/63625 (2022).

Download .ris file

Copy

Share Video

.

Copy