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An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
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An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
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September 20, 2022
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We constructed an untargeted metabolomic workflow that integrated XY-Meta and metaX together. In this protocol, we displayed how to use XY-Meta to generate a decoy spectral library from open access spectra reference, and then performed FDR control and used the metaX to quantitate the metabolites after identifying the metabolomics spectra.
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Li, D., Liang, J., Zhang, Y., Zhang, G. An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome. J. Vis. Exp. (187), e63625, doi:10.3791/63625 (2022).
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