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An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
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Biochemistry
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JoVE Journal Biochemistry
An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

English

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3,473 Views

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05:35 min

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September 20, 2022

DOI:

10.3791/63625-v

DOI:
10.3791/63625-v

•

05:35 min
September 20, 2022

•

3 Views
, , ,
1Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and MOE Key Laboratory of Tumor Molecular Biology, Institute of Life and Health Engineering, College of Life Science and Technology,Jinan University, 2Chi-Biotech Co. Ltd.

Summary

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We constructed an untargeted metabolomic workflow that integrated XY-Meta and metaX together. In this protocol, we displayed how to use XY-Meta to generate a decoy spectral library from open access spectra reference, and then performed FDR control and used the metaX to quantitate the metabolites after identifying the metabolomics spectra.

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Li, D., Liang, J., Zhang, Y., Zhang, G. An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome. J. Vis. Exp. (187), e63625, doi:10.3791/63625 (2022).

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