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Journal
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Immunology and Infection
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双室渗透支持系统中的犬肠类器官
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Canine Intestinal Organoids in a Dual-Chamber Permeable Support System
Please note that all translations are automatically generated.
Click here for the English version.
双室渗透支持系统中的犬肠类器官
DOI:
10.3791/63612-v
•
11:11 min
•
March 02, 2022
•
Vojtech Gabriel
,
Christopher Zdyrski
,
Dipak K. Sahoo
,
Kimberly Dao
,
Agnes Bourgois-Mochel
,
Todd Atherly
,
Marilyn N. Martinez
,
Donna A. Volpe
,
Jamie Kopper
,
Karin Allenspach
3
,
Jonathan P. Mochel
3
1
Department of Biomedical Sciences, College of Veterinary Medicine
,
Iowa State University
,
2
Department of Veterinary Clinical Sciences, College of Veterinary Medicine
,
Iowa State University
,
3
3D Health Solutions Inc.
,
4
Office of New Animal Drug Evaluation
,
Center for Veterinary Medicine, Food and Drug Administration
,
5
Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences
,
Center for Drug Evaluation and Research, Food and Drug Administration
Chapters
00:04
Introduction
01:05
Permeable Support Seeding
05:30
TEER Value Measurement
06:51
Experimental Layout
08:09
Results: Staining, Light Microscopy, and TEM Images of Canine Intestinal Organoid Monolayer
10:36
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个方案,描述了犬肠类器官在双室,可渗透的支持系统中的培养。描述了渗透性支撑物中的类器官接种、单层维持以及随后的药物渗透性实验。
Tags
Canine Intestinal Organoids
Dual-chamber Permeable Support System
Biomedical Research Models
Oral Permeability
Therapeutic Drug Candidates
In Vitro Tool
2D Cell Lines
3D Self-assembled Epithelial Structures
Adult Stem Cells
Monolayer Maintenance
Extracellular Matrix
Tight Junctions
Cell Differentiation
Nuclear Receptors
Caco-2
MDCK
Gastrointestinal Anatomy
Intestinal Microflora
Drug Permeability
Parallel Drug Development
Article
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