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Organoides intestinais caninos em um sistema de suporte permeável de câmara dupla
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March 02, 2022
DOI:
1Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, 33D Health Solutions Inc., 4Office of New Animal Drug Evaluation,Center for Veterinary Medicine, Food and Drug Administration, 5Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences,Center for Drug Evaluation and Research, Food and Drug Administration
Summary
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Aqui, apresentamos um protocolo descrevendo a cultura dos organoides intestinais caninos em um sistema de suporte permeável de câmara dupla. São descritos os suportes permeáveis, a manutenção da monocamada e os experimentos subsequentes de permeabilidade de drogas.
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Cite this Article
Gabriel, V., Zdyrski, C., Sahoo, D. K., Dao, K., Bourgois-Mochel, A., Atherly, T., Martinez, M. N., Volpe, D. A., Kopper, J., Allenspach, K., Mochel, J. P. Canine Intestinal Organoids in a Dual-Chamber Permeable Support System. J. Vis. Exp. (181), e63612, doi:10.3791/63612 (2022).
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