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Canine intestinale organoïden in een tweekamer permeabel ondersteuningssysteem
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11:11 min
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March 02, 2022
DOI:
1Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, 33D Health Solutions Inc., 4Office of New Animal Drug Evaluation,Center for Veterinary Medicine, Food and Drug Administration, 5Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences,Center for Drug Evaluation and Research, Food and Drug Administration
Summary
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Hier presenteren we een protocol dat de cultuur van canine intestinale organoïden beschrijft in een tweekamerig, permeabel ondersteuningssysteem. Organoïde zaaien in de doorlatende steunen, het onderhoud van de monolaag en de daaropvolgende experimenten met de permeabiliteit van geneesmiddelen worden beschreven.
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Cite this Article
Gabriel, V., Zdyrski, C., Sahoo, D. K., Dao, K., Bourgois-Mochel, A., Atherly, T., Martinez, M. N., Volpe, D. A., Kopper, J., Allenspach, K., Mochel, J. P. Canine Intestinal Organoids in a Dual-Chamber Permeable Support System. J. Vis. Exp. (181), e63612, doi:10.3791/63612 (2022).
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