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Konfokal mikroskopi til måling af tre tilstande af fusionsporedynamik i binyrekromaffinceller
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Chapters
- 00:04Introduction
- 01:07Bovine Chromaffin Cell Culture
- 02:52Transfection with Electroporation
- 04:18Preparation for Patch-Clamp Recording and Confocal Imaging
- 05:41Patch-Clamp Recording and Confocal Imaging
- 09:01Confocal Imaging Data Analysis
- 10:21Results: Whole-Cell Voltage-Clamp Recordings and Visualization of Fusion Events Under Confocal Microscope
- 11:52Conclusion
Denne protokol beskriver en konfokal billeddannelsesteknik til påvisning af tre fusionstilstande i bovin adrenal kromaffinceller. Disse fusionstilstande omfatter 1) tæt fusion (også kaldet kiss-and-run), der involverer fusionsporeåbning og lukning, 2) opholdsfusion, der involverer fusionsporeåbning og vedligeholdelse af den åbne pore, og 3) krympefusion, der involverer smeltet vesikelkrympning.
