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Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells
 

Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells

Article DOI: 10.3791/63569-v • 12:30 min • March 16th, 2022
March 16th, 2022 •
*1, *1, 1, 1
1National Institute of Neurological Disorders and Stroke
* These authors contributed equally

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This protocol describes a confocal imaging technique to detect three fusion modes in bovine adrenal chromaffin cells. These fusion modes include 1) close-fusion (also called kiss-and-run), involving fusion pore opening and closure, 2) stay-fusion, involving fusion pore opening and maintaining the opened pore, and 3) shrink-fusion, involving fused vesicle shrinkage.

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Confocal Microscopy Fusion Pore Dynamics Adrenal Chromaffin Cells Membrane Fusion Close Fusion Stay Fusion Shrink Fusion Patch-clamp Recording Transmitter Release Dynamics Visualization Kinetics Secretory Cells Primary Chromaffin Cell Culture Plasmid Transfection Efficiency Electrophysiological Recording Success Rate Intact Glands Fat Tissue Removal Locke's Solution Perfusion Enzyme Digestion Solution Injection Gland Unfolding
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