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Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells
 

Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells

Article DOI: 10.3791/63569-v 12:30 min March 16th, 2022
March 16th, 2022

章节

总结概括

This protocol describes a confocal imaging technique to detect three fusion modes in bovine adrenal chromaffin cells. These fusion modes include 1) close-fusion (also called kiss-and-run), involving fusion pore opening and closure, 2) stay-fusion, involving fusion pore opening and maintaining the opened pore, and 3) shrink-fusion, involving fused vesicle shrinkage.

Tags

Keywords: Confocal Microscopy Fusion Pore Dynamics Adrenal Chromaffin Cells Membrane Fusion Close Fusion Stay Fusion Shrink Fusion Patch-clamp Recording Primary Chromaffin Cell Culture Cell Isolation Cell Transfection
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