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共聚焦显微镜测量肾上腺嗜铬细胞融合孔隙动力学的三种模式
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells
DOI:

12:30 min

March 16, 2022

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Chapters

  • 00:04Introduction
  • 01:07Bovine Chromaffin Cell Culture
  • 02:52Transfection with Electroporation
  • 04:18Preparation for Patch-Clamp Recording and Confocal Imaging
  • 05:41Patch-Clamp Recording and Confocal Imaging
  • 09:01Confocal Imaging Data Analysis
  • 10:21Results: Whole-Cell Voltage-Clamp Recordings and Visualization of Fusion Events Under Confocal Microscope
  • 11:52Conclusion

Summary

Automatic Translation

该协议描述了一种共聚焦成像技术,用于检测牛肾上腺嗜铬细胞中的三种融合模式。这些融合模式包括1)闭合融合(也称为亲吻和运行),涉及融合孔隙开放和闭合,2)停留融合,涉及融合孔隙开放和维持开放孔,以及3)收缩融合,涉及融合囊泡收缩。

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