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Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
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JoVE Journal Neuroscience
Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

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8,504 Views

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08:53 min

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May 16, 2017

DOI:

10.3791/55676-v

DOI:
10.3791/55676-v

•

08:53 min
May 16, 2017

•

4 Views
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1Department of Physiology and Cell Biology, Faculty of Health Sciences,Ben-Gurion University of the Negev, 2The Zlotowski Center for Neuroscience,Ben-Gurion University of the Negev

Summary

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This protocol describes a method to sensitively measure the nucleocytoplasmic transport rate within motor neuron-like NSC-34 cells by quantifying the real-time change in the nuclear import of a NLS-NES-GFP protein.

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Cite this Article

Shani, T., Levy, M., Israelson, A. Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells. J. Vis. Exp. (123), e55676, doi:10.3791/55676 (2017).

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