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Co-Cultura de Organoides Epiteliais do Intestino Delgado Murine com Células Linfoides Inatas
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Immunology and Infection
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JoVE Journal Immunology and Infection
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Co-Cultura de Organoides Epiteliais do Intestino Delgado Murine com Células Linfoides Inatas

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4,777 Views

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08:22 min

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March 23, 2022

DOI:

10.3791/63554-v

DOI:
10.3791/63554-v

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08:22 min
March 23, 2022

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1Centre for Host-Microbiome Interactions,King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme,King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute,Cambridge University

Summary

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Este protocolo oferece instruções detalhadas para estabelecer organoides do intestino delgado murine, isolar células linfoides tipo 1 da lamina propria do intestino delgado murina e estabelecer coculturas tridimensionais (3D) entre ambos os tipos de células para estudar interações bidirecionais entre células epiteliais intestinais e células linfoides inatas tipo 1.

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Read, E., Jowett, G. M., Coman, D., Neves, J. F. Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells. J. Vis. Exp. (181), e63554, doi:10.3791/63554 (2022).

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