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Kokultur von murinen Dünndarm-Epithelorganoiden mit angeborenen lymphatischen Zellen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
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Kokultur von murinen Dünndarm-Epithelorganoiden mit angeborenen lymphatischen Zellen

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4,777 Views

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08:22 min

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March 23, 2022

DOI:

10.3791/63554-v

DOI:
10.3791/63554-v

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08:22 min
March 23, 2022

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14 Views
, , ,
1Centre for Host-Microbiome Interactions,King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme,King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute,Cambridge University

Summary

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Dieses Protokoll bietet detaillierte Anweisungen zur Etablierung von murinen Dünndarmorganoiden, zur Isolierung angeborener Lymphzellen des Typs 1 aus der murinen Dünndarmlamina propria und zur Etablierung von 3-dimensionalen (3D) Kokulturen zwischen beiden Zelltypen, um bidirektionale Wechselwirkungen zwischen Darmepithelzellen und angeborenen lymphatischen Typ-1-Zellen zu untersuchen.

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Read, E., Jowett, G. M., Coman, D., Neves, J. F. Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells. J. Vis. Exp. (181), e63554, doi:10.3791/63554 (2022).

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