Journal
/
면역학 및 감염병학
/
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

4,776 Views

•

08:22 min

•

March 23, 2022

DOI:

10.3791/63554-v

DOI:
10.3791/63554-v

•

08:22 min
March 23, 2022

•

14 Views
, , ,
1Centre for Host-Microbiome Interactions,King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme,King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute,Cambridge University

Summary

Automatically generated

This protocol offers detailed instructions for establishing murine small intestine organoids, isolating type-1 innate lymphoid cells from the murine small intestine lamina propria, and establishing 3-dimensional (3D) co-cultures between both cell types to study bi-directional interactions between intestinal epithelial cells and type-1 innate lymphoid cells.

Explore More Videos

Co-cultureMurineSmall IntestineEpithelial OrganoidsInnate Lymphoid CellsIn Vitro ModelIntestinal HomeostasisInflammationILC1CD44Gastrointestinal DiseasesThermal Cross-linkingBasal Extracellular MatrixAdvanced DMEM F12PBS2Centrifugation

Read Article

Cite this Article

Read, E., Jowett, G. M., Coman, D., Neves, J. F. Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells. J. Vis. Exp. (181), e63554, doi:10.3791/63554 (2022).

Download .ris file

Copy

Share Video

.

Copy