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Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

4,776 Views

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08:22 min

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March 23, 2022

DOI:

10.3791/63554-v

DOI:
10.3791/63554-v

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08:22 min
March 23, 2022

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14 Views
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1Centre for Host-Microbiome Interactions,King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme,King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute,Cambridge University

Summary

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This protocol offers detailed instructions for establishing murine small intestine organoids, isolating type-1 innate lymphoid cells from the murine small intestine lamina propria, and establishing 3-dimensional (3D) co-cultures between both cell types to study bi-directional interactions between intestinal epithelial cells and type-1 innate lymphoid cells.

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Read, E., Jowett, G. M., Coman, D., Neves, J. F. Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells. J. Vis. Exp. (181), e63554, doi:10.3791/63554 (2022).

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