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Isolamento de Células Linfoides Inatas do Grupo 2 da Mucosa Nasal de Camundongos para Detecção da Expressão de CD226
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Isolamento de Células Linfoides Inatas do Grupo 2 da Mucosa Nasal de Camundongos para Detecção da Expressão de CD226

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1,591 Views

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08:30 min

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May 10, 2022

DOI:

10.3791/63525-v

DOI:
10.3791/63525-v

•

08:30 min
May 10, 2022

•

5 Views
, , , , , ,
1Otolaryngological Department of Tangdu Hospital,Fourth Military Medical University, 2Institute of Medical Research,Northwestern Polytechnical University, 3Orthopedic Department of Tangdu Hospital,Fourth Military Medical University, 4Department of Immunology,Fourth Military Medical University

Summary

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As células linfoides inatas do grupo 2 (ILC2s), implicadas na inflamação do tipo 2, participam principalmente da resposta à infecção por helmintos, doenças alérgicas, homeostase metabólica e reparo tecidual. Neste estudo, é demonstrado um procedimento para isolar ILC2s da mucosa nasal murina e detectar a expressão de CD226.

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Xie, Y., Zhang, Y., Liu, Y., Wang, Y., Cheng, K., Zhuang, R., Bian, K. Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226. J. Vis. Exp. (183), e63525, doi:10.3791/63525 (2022).

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