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Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

1,596 Views

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08:30 min

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May 10, 2022

DOI:

10.3791/63525-v

DOI:
10.3791/63525-v

•

08:30 min
May 10, 2022

•

5 Views
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1Otolaryngological Department of Tangdu Hospital,Fourth Military Medical University, 2Institute of Medical Research,Northwestern Polytechnical University, 3Orthopedic Department of Tangdu Hospital,Fourth Military Medical University, 4Department of Immunology,Fourth Military Medical University

Summary

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Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated.

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Innate Lymphoid CellsILC2Nasal MucosaMouseFlow CytometryAllergic RhinitisOvalbuminCell Isolation

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Xie, Y., Zhang, Y., Liu, Y., Wang, Y., Cheng, K., Zhuang, R., Bian, K. Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226. J. Vis. Exp. (183), e63525, doi:10.3791/63525 (2022).

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