Journal
/
Imunologia e Infecção
/
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
JoVE Journal
Imunologia e Infecção
Author Produced
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

1,590 Views

•

08:30 min

•

May 10, 2022

DOI:

10.3791/63525-v

DOI:
10.3791/63525-v

•

08:30 min
May 10, 2022

•

5 Views
, , , , , ,
1Otolaryngological Department of Tangdu Hospital,Fourth Military Medical University, 2Institute of Medical Research,Northwestern Polytechnical University, 3Orthopedic Department of Tangdu Hospital,Fourth Military Medical University, 4Department of Immunology,Fourth Military Medical University

Summary

Automatically generated

Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated.

Explore More Videos

Innate Lymphoid CellsILC2Nasal MucosaMouseFlow CytometryAllergic RhinitisOvalbuminCell Isolation

Read Article

Cite this Article

Xie, Y., Zhang, Y., Liu, Y., Wang, Y., Cheng, K., Zhuang, R., Bian, K. Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226. J. Vis. Exp. (183), e63525, doi:10.3791/63525 (2022).

Download .ris file

Copy

Share Video

.

Copy