ジャーナル
/
免疫学と感染
/
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
JoVE Journal
免疫学と感染
Author Produced
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

DOI:
10.3791/63525-v

•

08:30 min

•

May 10, 2022

•

, , , , , ,
1Otolaryngological Department of Tangdu Hospital,Fourth Military Medical University, 2Institute of Medical Research,Northwestern Polytechnical University, 3Orthopedic Department of Tangdu Hospital,Fourth Military Medical University, 4Department of Immunology,Fourth Military Medical University

章

  • 00:05Introduction
  • 01:00Murine Allergic Rhinitis Model Establishment
  • 02:03Isolation of Mononuclear Cells from the Nasal Mucosa
  • 04:52Surface Staining for Flow Cytometry Analysis
  • 06:08Results: Ovalbumin-Induced Murine Model Development to Explore the Role of ILC2s in Allergic Rhinitis
  • 07:27Conclusion

概要

自動翻訳

自動翻訳

Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated.

タグ

Innate Lymphoid CellsILC2Nasal MucosaMouseFlow CytometryAllergic RhinitisOvalbuminCell Isolation

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Isolation of Mouse Lung Dendritic Cells

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Isolation of Lymphocytes from Mouse Genital Tract Mucosa

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue

Read Article