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Developmental Biology
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活斑马鱼幼虫的眼睛去除以检查视觉系统的神经支配依赖性生长和发育
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Developmental Biology
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JoVE Journal
Developmental Biology
Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System
Please note that all translations are automatically generated.
Click here for the English version.
活斑马鱼幼虫的眼睛去除以检查视觉系统的神经支配依赖性生长和发育
DOI:
10.3791/63509-v
•
09:00 min
•
February 11, 2022
•
Olivia L. Hagen
,
Yehyun Kim
,
Elaine Kushkowski
2
,
Hannah Rouse
,
Kara L. Cerveny
1
Department of Biology
,
Reed College
,
2
Committee on Development, Regeneration, and Stem Cell Biology
,
University of Chicago
,
3
Department of Cell and Developmental Biology
,
University College London
Chapters
00:04
Introduction
00:49
Electrolytically Sharpened Tungsten Needles
01:45
Preparing Larvae for Surgery
03:08
Eye-Removal and Postoperative Care
04:27
Dissecting Larvae to Reveal Brains
06:03
Mounting and Imaging
07:01
Results: Vertebrate Visual System Development in Zebrafish Larvae
08:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文解释了如何手术从活斑马鱼幼虫身上摘除眼睛,作为研究视网膜输入如何影响视神经结构生长和发育的第一步。此外,本文还提供了有关幼虫麻醉、固定和脑解剖的信息,然后是免疫组化和共聚焦成像。
Tags
Eye Removal
Zebrafish Larvae
Innervation-dependent Growth
Visual System
Retinal Input
Optic Tectum
Neural Development
Regeneration
Degeneration
Surgical Technique
Anesthesia
Immobilization
Agarose
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