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Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System
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Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System
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February 11, 2022
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The article explains how to surgically remove eyes from living zebrafish larvae as the first step toward investigating how retinal input influences optic tectum growth and development. In addition, the article provides information about larval anesthetization, fixation, and brain dissection, followed by immunohistochemistry and confocal imaging.
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Hagen, O. L., Kim, Y., Kushkowski, E., Rouse, H., Cerveny, K. L. Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System. J. Vis. Exp. (180), e63509, doi:10.3791/63509 (2022).
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