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Administración intraductal y visualización por rayos X de la solución ablativa a base de etanol para la prevención y el tratamiento local del cáncer de mama en modelos de ratón
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models
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Administración intraductal y visualización por rayos X de la solución ablativa a base de etanol para la prevención y el tratamiento local del cáncer de mama en modelos de ratón

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4,148 Views

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13:43 min

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April 01, 2022

DOI:

10.3791/63457-v

DOI:
10.3791/63457-v

•

13:43 min
April 01, 2022

•

4 Views
, , , , , , , , , ,
1Precision Health Program,Michigan State University, 2Department of Radiology, College of Human Medicine,Michigan State University, 3Department of Pharmacology & Toxicology, College of Veterinary Medicine,Michigan State University, 4College of Osteopathic Medicine,Michigan State University, 5Advanced Materials Characterization Laboratory/Materials Research Center,Missouri University of Science and Technology, 6Institute for Quantitative (IQ) Health Science and Engineering Advanced Molecular Imaging Facility,Michigan State University, 7Department of Obstetrics Gynecology and Reproductive Biology, College of Human Medicine,Michigan State University, 8Institute for Quantitative (IQ) Health Science and Engineering,Michigan State University, 9Van Andel Research Institute, 10Miltenyi Biotec, 11Veterinary Diagnostic Laboratory, College of Veterinary Medicine,Michigan State University

Summary

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Se describe un método de inyección intraductal de reactivos para una solución ablativa a base de etanol al árbol ductal mamario de ratón para imágenes in vivo y prevención del cáncer de mama. La inyección directamente en la abertura del pezón permite dirigirse a las células epiteliales mamarias con un daño mínimo en el tejido colateral.

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Kenyon, E., Zaluzec, E. K., Powell, K., Volk, M., Chakravarty, S., Hix, J., Arora, R., Westerhuis, J. J., Kiupel, M., Shapiro, E. M., Sempere, L. F. Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models. J. Vis. Exp. (182), e63457, doi:10.3791/63457 (2022).

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