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Administration intracanalaire et visualisation aux rayons X d’une solution ablative à base d’éthanol pour la prévention et le traitement local du cancer du sein dans des modèles murins
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models
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Administration intracanalaire et visualisation aux rayons X d’une solution ablative à base d’éthanol pour la prévention et le traitement local du cancer du sein dans des modèles murins

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4,148 Views

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13:43 min

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April 01, 2022

DOI:

10.3791/63457-v

DOI:
10.3791/63457-v

•

13:43 min
April 01, 2022

•

4 Views
, , , , , , , , , ,
1Precision Health Program,Michigan State University, 2Department of Radiology, College of Human Medicine,Michigan State University, 3Department of Pharmacology & Toxicology, College of Veterinary Medicine,Michigan State University, 4College of Osteopathic Medicine,Michigan State University, 5Advanced Materials Characterization Laboratory/Materials Research Center,Missouri University of Science and Technology, 6Institute for Quantitative (IQ) Health Science and Engineering Advanced Molecular Imaging Facility,Michigan State University, 7Department of Obstetrics Gynecology and Reproductive Biology, College of Human Medicine,Michigan State University, 8Institute for Quantitative (IQ) Health Science and Engineering,Michigan State University, 9Van Andel Research Institute, 10Miltenyi Biotec, 11Veterinary Diagnostic Laboratory, College of Veterinary Medicine,Michigan State University

Summary

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Une méthode d’injection intracanalaire de réactifs pour une solution ablative à base d’éthanol dans l’arbre canalaire mammaire de la souris pour l’imagerie in vivo et la prévention du cancer du sein est décrite. L’injection directement dans l’ouverture du mamelon permet de cibler les cellules épithéliales mammaires avec un minimum de dommages aux tissus collatéraux.

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Kenyon, E., Zaluzec, E. K., Powell, K., Volk, M., Chakravarty, S., Hix, J., Arora, R., Westerhuis, J. J., Kiupel, M., Shapiro, E. M., Sempere, L. F. Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models. J. Vis. Exp. (182), e63457, doi:10.3791/63457 (2022).

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