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Somministrazione intraduttale e visualizzazione a raggi X della soluzione ablativa a base di etanolo per la prevenzione e il trattamento locale del cancro al seno in modelli murini
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models
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Somministrazione intraduttale e visualizzazione a raggi X della soluzione ablativa a base di etanolo per la prevenzione e il trattamento locale del cancro al seno in modelli murini

italiano

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4,138 Views

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13:43 min

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April 01, 2022

DOI:

10.3791/63457-v

DOI:
10.3791/63457-v

•

13:43 min
April 01, 2022

•

4 Views
, , , , , , , , , ,
1Precision Health Program,Michigan State University, 2Department of Radiology, College of Human Medicine,Michigan State University, 3Department of Pharmacology & Toxicology, College of Veterinary Medicine,Michigan State University, 4College of Osteopathic Medicine,Michigan State University, 5Advanced Materials Characterization Laboratory/Materials Research Center,Missouri University of Science and Technology, 6Institute for Quantitative (IQ) Health Science and Engineering Advanced Molecular Imaging Facility,Michigan State University, 7Department of Obstetrics Gynecology and Reproductive Biology, College of Human Medicine,Michigan State University, 8Institute for Quantitative (IQ) Health Science and Engineering,Michigan State University, 9Van Andel Research Institute, 10Miltenyi Biotec, 11Veterinary Diagnostic Laboratory, College of Veterinary Medicine,Michigan State University

Summary

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Viene descritto un metodo di iniezione intraduttale di reagenti per una soluzione ablativa a base di etanolo all'albero duttale mammario di topo per l'imaging in vivo e la prevenzione del cancro al seno. L'iniezione direttamente nell'apertura del capezzolo consente di colpire le cellule epiteliali mammarie con un danno tissutale collaterale minimo.

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Kenyon, E., Zaluzec, E. K., Powell, K., Volk, M., Chakravarty, S., Hix, J., Arora, R., Westerhuis, J. J., Kiupel, M., Shapiro, E. M., Sempere, L. F. Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models. J. Vis. Exp. (182), e63457, doi:10.3791/63457 (2022).

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