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Journal
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Biochemistry
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使用透射电子显微镜定量骨骼肌纤维中的亚细胞糖原分布
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用透射电子显微镜定量骨骼肌纤维中的亚细胞糖原分布
DOI:
10.3791/63347-v
•
08:32 min
•
February 07, 2022
•
Rasmus Jensen
,
Niels Ørtenblad
,
Cristiano di Benedetto
,
Klaus Qvortrup
,
Joachim Nielsen
1
Research center for applied health science
,
University College South Denmark
,
2
Department of Sports Science and Clinical Biomechanics
,
University of Southern Denmark
,
3
Department of Biomedical Sciences, Core Facility for Integrated Microscopy
,
University of Copenhagen
Chapters
00:04
Introduction
00:55
Primary Fixation, Post-Fixation, and Embedding
02:46
Ultrathin Sectioning of Fibers and Contrasting
03:57
Imaging
04:59
Image Analyses
07:33
Results: Glycogen Content in Fiber
07:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
改进的固定后程序增加了组织中糖原颗粒的对比度。本文提供了一个分步方案,描述了如何处理组织,进行成像,并使用立体学方法获得骨骼肌中纤维类型特异性亚细胞糖原分布的无偏和定量数据。
Tags
Glycogen Distribution
Subcellular
Skeletal Muscle Fibers
Transmission Electron Microscopy
High Resolution
Antibody-free Staining
Potassium Ferrocyanide
Glutaraldehyde Fixation
Osmium Tetroxide
Epoxy Resin Embedding
Ultramicrotomy
Semi-thin Sectioning
Light Microscopy
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