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Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy
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Bioquímica
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JoVE Journal Bioquímica
Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

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08:32 min

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February 07, 2022

DOI:

10.3791/63347-v

DOI:
10.3791/63347-v

•

08:32 min
February 07, 2022

•

13 Views
, , , ,
1Research center for applied health science,University College South Denmark, 2Department of Sports Science and Clinical Biomechanics,University of Southern Denmark, 3Department of Biomedical Sciences, Core Facility for Integrated Microscopy,University of Copenhagen

Summary

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A modified post-fixation procedure increases the contrast of glycogen particles in tissue. This paper provides a step-by-step protocol describing how to handle the tissue, conduct the imaging, and use stereological methods to obtain unbiased and quantitative data on fiber type-specific subcellular glycogen distribution in skeletal muscle.

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Jensen, R., Ørtenblad, N., di Benedetto, C., Qvortrup, K., Nielsen, J. Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy. J. Vis. Exp. (180), e63347, doi:10.3791/63347 (2022).

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