Journal
/
Biochemie
/
Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

DOI:
10.3791/63347-v

•

08:32 min

•

February 07, 2022

•

, , , ,
1Research center for applied health science,University College South Denmark, 2Department of Sports Science and Clinical Biomechanics,University of Southern Denmark, 3Department of Biomedical Sciences, Core Facility for Integrated Microscopy,University of Copenhagen

Kapitel

  • 00:04Introduction
  • 00:55Primary Fixation, Post-Fixation, and Embedding
  • 02:46Ultrathin Sectioning of Fibers and Contrasting
  • 03:57Imaging
  • 04:59Image Analyses
  • 07:33Results: Glycogen Content in Fiber
  • 07:59Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

A modified post-fixation procedure increases the contrast of glycogen particles in tissue. This paper provides a step-by-step protocol describing how to handle the tissue, conduct the imaging, and use stereological methods to obtain unbiased and quantitative data on fiber type-specific subcellular glycogen distribution in skeletal muscle.

Tags

Glycogen DistributionSubcellularSkeletal Muscle FibersTransmission Electron MicroscopyHigh ResolutionAntibody-free StainingPotassium FerrocyanideGlutaraldehyde FixationOsmium TetroxideEpoxy Resin EmbeddingUltramicrotomySemi-thin SectioningLight Microscopy

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

Analysis of β-Amyloid-induced Abnormalities on Fibrin Clot Structure by Spectroscopy and Scanning Electron Microscopy

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Author Spotlight: Deciphering the Mysteries of Skeletal Muscle Fiber Types Using the MyDoBID Technique

Read Article