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可视化 果蝇 胚胎中含脂质细胞器的细胞骨架依赖性运输
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos
DOI:

08:55 min

December 13, 2021

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Chapters

  • 00:05Introduction
  • 00:40Injection Needle Preparation
  • 01:26Injection Solution Preparation and Needle Loading
  • 02:08Embryo Preparation for Microinjection
  • 04:34Microinjecting Embryos
  • 05:51Imaging Embryos
  • 06:38Results: Representative Results for Organelle Dependent Trafficking in Drosophila Embryos
  • 08:14Conclusion

Summary

Automatic Translation

在早期的 果蝇 胚胎中,许多细胞器是运动的。原则上,它们 可以通过特定的荧光 探针进行现场成像,但蛋壳会阻止直接应用于胚胎。该协议描述了如何 通过 显微注射引入这种探针,然后 通过 粒子图像测速分析本体细胞器运动。

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