This content is Free Access.
Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos
2,017 Views
•
08:55 min
•
December 13, 2021
DOI:
Summary
Automatically generated
In the early Drosophila embryo, many organelles are motile. In principle, they can be imaged live via specific fluorescent probes, but the eggshell prevents direct application to the embryo. This protocol describes how to introduce such probes via microinjection, and then analyze bulk organelle motion via particle image velocimetry.
Read Article
Cite this Article
Kilwein, M. D., Welte, M. A. Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos. J. Vis. Exp. (178), e63291, doi:10.3791/63291 (2021).
Copy