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Journal
/
Immunology and Infection
/
Isolation of Primary Mouse Lung Endothelial Cells
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Isolation of Primary Mouse Lung Endothelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
Isolation of Primary Mouse Lung Endothelial Cells
DOI:
10.3791/63253-v
•
06:41 min
•
November 10, 2021
•
Erika Wong
2
,
Nina Nguyen
,
Judith Hellman
1
Department of Pediatrics, Division of Critical Care
,
UCSF Benioff Children’s Hospital
,
2
Department of Anesthesia and Perioperative Care
,
University of California, San Francisco
Chapters
00:05
Introduction
00:36
Precoating of Anti-Rat Magnetic Beads
01:34
Isolation and Plating of the Lung Cells
03:38
Primary Immunobead Isolation and Culturing of Endothelial Cells
05:24
Results: Morphology and Analysis of Murine Lung Endothelial Cells
06:13
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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italiano (Italian)
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português (Portuguese)
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Automatic Translation
In this article, primary lung endothelial cells were isolated and cultured from neonatal mice.
Tags
Primary Mouse Lung
Endothelial Cells
Isolation
Cell Culture
CD31
CD102
Immunobeads
Collagenase
HBSS
DMEM
Cell Strainer
Trituration
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