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Isolation of Primary Mouse Lung Endothelial Cells
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Isolation of Primary Mouse Lung Endothelial Cells

Isolation of Primary Mouse Lung Endothelial Cells

DOI:
10.3791/63253-v

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06:41 min

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November 10, 2021

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, ,
1Department of Pediatrics, Division of Critical Care,UCSF Benioff Children’s Hospital, 2Department of Anesthesia and Perioperative Care,University of California, San Francisco

Chapters

  • 00:05Introduction
  • 00:36Precoating of Anti-Rat Magnetic Beads
  • 01:34Isolation and Plating of the Lung Cells
  • 03:38Primary Immunobead Isolation and Culturing of Endothelial Cells
  • 05:24Results: Morphology and Analysis of Murine Lung Endothelial Cells
  • 06:13Conclusion

Summary

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In this article, primary lung endothelial cells were isolated and cultured from neonatal mice.

Tags

Primary Mouse LungEndothelial CellsIsolationCell CultureCD31CD102ImmunobeadsCollagenaseHBSSDMEMCell StrainerTrituration

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