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Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

English

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16,374 Views

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09:33 min

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April 05, 2017

DOI:

10.3791/55465-v

DOI:
10.3791/55465-v

•

09:33 min
April 05, 2017

•

24 Views
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1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research,National Institute of Environmental Health Sciences, NIH

Summary

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We describe a method for generating Precision-cut Lung Slices (PCLS) and immunostaining them to visualize the localization of various immune cell types in the lung. Our protocol can be extended to visualize the location and function of many different cell types under a variety of conditions.

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Cite this Article

Lyons-Cohen, M. R., Thomas, S. Y., Cook, D. N., Nakano, H. Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells. J. Vis. Exp. (122), e55465, doi:10.3791/55465 (2017).

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