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炎症性半月酶诱导的人单核细胞来源巨噬细胞邻近性的可视化
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Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages
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炎症性半月酶诱导的人单核细胞来源巨噬细胞邻近性的可视化

DOI:
10.3791/63162-v

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08:41 min

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April 06, 2022

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1Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology,Baylor College of Medicine

Chapters

  • 00:04Introduction
  • 00:51Preparation of Cells for Electroporation
  • 02:11Nucleofection of Caspase BiFC Components into Human Monocyte-Derived Macrophages
  • 04:41Treatment of Transfected MDM and Caspase BiFC Data Acquisition
  • 06:37Results: Differentiation of CD14-Monocytes and Transfection of Human Monocyte-Derived Macrophages
  • 08:12Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了从人血液样本中获取单核细胞来源巨噬细胞（MDM）的工作流程，一种在不损害细胞活力和行为的情况下将炎症半胱天冬酶双分子荧光互补（BiFC）报告基因有效引入人MDM的简单方法，以及一种基于成像的方法来测量活细胞中的炎症半胱天冬酶活化。

Tags

Inflammatory CaspasesProximityHuman Monocyte-derived MacrophagesVisualizationMolecular-levelPrimary Immune CellsOligomerizationMicroscopic MethodologiesTrypsin-EDTAPBSCell NumberHemocytometerReporter PlasmidCaspase BiFC PlasmidsNucleofectionElectroporation

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