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Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
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Immunology and Infection
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JoVE Journal Immunology and Infection
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

English

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22,799 Views

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10:43 min

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June 22, 2017

DOI:

10.3791/55781-v

DOI:
10.3791/55781-v

•

10:43 min
June 22, 2017

•

25 Views
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1Inserm U 1227, CNRS UMR 7225, 2Sorbonne Universités,UPMC, University of Paris, 3Institut du Cerveau et de la Moelle épinière, ICM, 4AP-HP, Hôpital de la Pitié Salpêtrière;

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This protocol provides an analysis of the macrophage subpopulations in the adult mouse central nervous system by flow cytometry and is helpful for the study of multiple markers expressed by these cells.

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MicrogliaMonocyte-derived MacrophagesCentral Nervous SystemFlow CytometryNeuroimmunologyMacrophage SubpopulationsCell Marker AnalysisCell IsolationSpinal CordBrain

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Martin, E., El-Behi, M., Fontaine, B., Delarasse, C. Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry. J. Vis. Exp. (124), e55781, doi:10.3791/55781 (2017).

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