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Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages
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Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages
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April 06, 2022
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This protocol describes the workflow to obtain monocytes-derived macrophages (MDM) from human blood samples, a simple method to efficiently introduce inflammatory caspase Bimolecular Fluorescence Complementation (BiFC) reporters into human MDM without compromising cell viability and behavior, and an imaging-based approach to measure inflammatory caspase activation in living cells.
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Bolívar, B. E., Bouchier-Hayes, L. Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages. J. Vis. Exp. (182), e63162, doi:10.3791/63162 (2022).
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