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Pluripotent Kök Hücrelerden Elde Edilen Kendi Kendine Monte Edilen İnsan Kalbi Organoidlerinin Üretilmesi
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Biochemistry
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JoVE Journal Biochemistry
Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells
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Pluripotent Kök Hücrelerden Elde Edilen Kendi Kendine Monte Edilen İnsan Kalbi Organoidlerinin Üretilmesi

Türkçe

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8,407 Views

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08:56 min

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September 15, 2021

DOI:

10.3791/63097-v

DOI:
10.3791/63097-v

•

08:56 min
September 15, 2021

•

17 Views
, , , ,
1Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology,Michigan State University, 2Department of Biomedical Engineering, College of Engineering,Michigan State University

Summary

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Burada, insan pluripotent kök hücrelerini kendi kendine örgütlenerek verimli bir şekilde kullanarak gelişimsel olarak ilgili insan kalbi organoidleri (hSO'lar) oluşturmak için bir protokol açıklıyoruz. Protokol, gelişimsel ipuçlarının sıralı aktivasyonuna dayanır ve son derece karmaşık, işlevsel olarak ilgili insan kalp dokuları üretir.

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Lewis-Israeli, Y. R., Volmert, B. D., Gabalski, M. A., Huang, A. R., Aguirre, A. Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells. J. Vis. Exp. (175), e63097, doi:10.3791/63097 (2021).

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