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Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells
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生物化学
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Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

8,401 Views

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08:56 min

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September 15, 2021

DOI:

10.3791/63097-v

DOI:
10.3791/63097-v

•

08:56 min
September 15, 2021

•

17 Views
, , , ,
1Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology,Michigan State University, 2Department of Biomedical Engineering, College of Engineering,Michigan State University

Summary

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Here, we describe a protocol to create developmentally relevant human heart organoids (hHOs) efficiently using human pluripotent stem cells by self-organization. The protocol relies on the sequential activation of developmental cues and produces highly complex, functionally relevant human heart tissues.

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Lewis-Israeli, Y. R., Volmert, B. D., Gabalski, M. A., Huang, A. R., Aguirre, A. Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells. J. Vis. Exp. (175), e63097, doi:10.3791/63097 (2021).

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