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Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

DOI:
10.3791/63097-v

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08:56 min

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September 15, 2021

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, , , ,
1Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology,Michigan State University, 2Department of Biomedical Engineering, College of Engineering,Michigan State University

Bölümler

  • 00:04Introduction
  • 00:59Generation of 3D Self-Assembling Human Heart Organoids
  • 02:51Human Heart Organoid (hHO) Differentiation
  • 05:33Organoid Analysis
  • 06:03Results: Self-organizing hHO
  • 08:02Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we describe a protocol to create developmentally relevant human heart organoids (hHOs) efficiently using human pluripotent stem cells by self-organization. The protocol relies on the sequential activation of developmental cues and produces highly complex, functionally relevant human heart tissues.

Etiketler

Self-assemblingHuman HeartOrganoidsPluripotent Stem Cells3D StructureDeveloping HeartCardiovascular DiseasesPharmacological TreatmentsHigh ThroughputCongenital Heart DefectsEmbryoid BodiesCell CultureDifferentiationHPSCDPBSACUTAPSC MediumThiasCell SuspensionCell CounterHemo CytometerRound Bottom Ultra Low Attachment 96 Well PlateRPMI 1640Insulin Free B27 Supplement

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