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Gerando organoides cardíacos humanos auto-montados derivados de células-tronco pluripotentes
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Biochemistry
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JoVE Journal Biochemistry
Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

Gerando organoides cardíacos humanos auto-montados derivados de células-tronco pluripotentes

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8,407 Views

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08:56 min

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September 15, 2021

DOI:

10.3791/63097-v

DOI:
10.3791/63097-v

•

08:56 min
September 15, 2021

•

17 Views
, , , ,
1Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology,Michigan State University, 2Department of Biomedical Engineering, College of Engineering,Michigan State University

Summary

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Aqui, descrevemos um protocolo para criar organoides cardíacos humanos relevantes de desenvolvimento (hHOs) usando eficientemente células-tronco pluripotentes humanas por auto-organização. O protocolo conta com a ativação sequencial de pistas de desenvolvimento e produz tecidos cardíacos humanos altamente complexos e funcionalmente relevantes.

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Lewis-Israeli, Y. R., Volmert, B. D., Gabalski, M. A., Huang, A. R., Aguirre, A. Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells. J. Vis. Exp. (175), e63097, doi:10.3791/63097 (2021).

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