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Paramagnetische relaxatieverbetering voor het detecteren en karakteriseren van zelfassociaties van intrinsiek ongeordende eiwitten
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
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Paramagnetische relaxatieverbetering voor het detecteren en karakteriseren van zelfassociaties van intrinsiek ongeordende eiwitten

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1,519 Views

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07:24 min

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September 23, 2021

DOI:

10.3791/63057-v

DOI:
10.3791/63057-v

•

07:24 min
September 23, 2021

•

8 Views
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1Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute,The University of Texas Health Science Center at San Antonio

Summary

Automatically generated

Een protocol voor de toepassing van paramagnetische relaxatieverbetering NMR-spectroscopie om zwakke en voorbijgaande inter- en intramoleculaire interacties in intrinsiek ongeordende eiwitten te detecteren, wordt gepresenteerd.

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Cite this Article

Johnson, C. N., Libich, D. S. Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins. J. Vis. Exp. (175), e63057, doi:10.3791/63057 (2021).

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