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Potenziamento del rilassamento paramagnetico per rilevare e caratterizzare le auto-associazioni di proteine intrinsecamente disordinate
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Biochemistry
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JoVE Journal Biochemistry
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
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Potenziamento del rilassamento paramagnetico per rilevare e caratterizzare le auto-associazioni di proteine intrinsecamente disordinate

italiano

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1,517 Views

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07:24 min

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September 23, 2021

DOI:

10.3791/63057-v

DOI:
10.3791/63057-v

•

07:24 min
September 23, 2021

•

8 Views
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1Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute,The University of Texas Health Science Center at San Antonio

Summary

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Viene presentato un protocollo per l'applicazione della spettroscopia NMR di potenziamento del rilassamento paramagnetico per rilevare interazioni inter- e intra-molecolari deboli e transitorie in proteine intrinsecamente disordinate.

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Cite this Article

Johnson, C. N., Libich, D. S. Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins. J. Vis. Exp. (175), e63057, doi:10.3791/63057 (2021).

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